![]() ![]() However, this loop extrusion on a bare DNA answers only a part of the whole puzzle of 3D organization of chromosome ![]() The dynamic visualization of the loop formation flooded us with details like the velocity and step size of the condensin motor, asymmetric nature of the loop extrusion, and the size of the loops which disclosed the underlying mechanisms of condensin. We recently showed that condensin compacts DNA upon ATP hydrolysis (Fig.1) and the underlying mechanism of DNA compaction is by extruding loops on DNA in a progressive manner. We employ various single-molecule assays like magnetic tweezers, fluorescence microscopy, and atomic force microscopy to elucidate the mechanical properties of SMC proteins. Structural maintenance of chromosome (SMC) is a class of protein complexes that organizes DNA into the sister chromatids in the mitotic phase of a cell cycle. ![]() ![]() Loop extrusion by condensin: from bare DNA towards chromosome organization ![]()
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